The air-dried fresh fruit hulls of Garcinia mangostana Linn. had been removed with 70percent MeOH, and then partitioned in to the -BuOH portions. Additionally, three significant phenolic elements associated with their particular anti-oxidant tasks had been purified by silica serum column chromatography and Sephadex LH-20 then defined as P1 (1, 3, 6, 7-tetrahydroxy-2, 8-(3-methyl-2-butenyl)), P2 [1, 3, 6-trihydroxy-7-methoxy-2, 8-(3-methyl-2-butenyl) xanthone] and P3 (epicatechin) using UV–visible spectrophotometry, IR spectrophotometry and NMR spectroscopy, correspondingly. The antioxidant activities of three significant phenolics had been assessed making use of different examinations, such as the free radical scavenging ability and complete anti-oxidant task in a linoleic acid peroxidation. These three phenolic substances exhibited various antioxidant tasks within these anti-oxidant examinations. The hydroxyl radical and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capabilities as well as the activity against linoleic acid peroxidation of P1 were greater than those of P2 and P3, although the superoxide anion radical scavenging task of P3 was higher than compared to P1, but was near that P2 or α-tocopherol. An activity-guided isolation and purification process had been always recognize the elements showing the strong DPPH radical scavenging activity from G. mangostana Linn.
- Garcinia mangostana Linn.;
- Antioxidant activity